On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25. You are supplied with a filter that selects a wavelength of 520nm. Required:
a. What is the limit of resolution on this microscope?
b. Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?

- colony: mass of single-type cells formed from single cell/small chain; pair or small cluster of cells; visible to naked eye

- colony forming unit: not visible to eye, original single cell or small chain/pair or small cluster of cells FROM WHICH colony forms

- Streaking plate: producing isolated colonies upon incubation using streak plate methods in agar

- Quadrant streak: used when sample is of high density

- Zigzag pattern: used when sample is of low density