The nitrite ion is involved in the biochemical nitrogen cycle. You can determine the nitrite ion content of a sample using spectrophotometry by first using several reactions to form a colored compound from the ion. The following data were collected.
Nitrite Ion concentration (M) Absorbance of solution at 550 nm
2.00 x10^-6 M 0.065
6.00 x10^-6 M 0.205
10.00 x10^-6 0.338
14.00 x10^-6 0.474
18.00 x10^-6 0.598
Unknown 0.402
Required:
a. Construct a calibration plot, and determine the slope and intercept.
b. What is the nitrite ion concentration in the unknown solution?
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