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Chemistry, 01.10.2019 05:00 nyceastcoast
One molecule of affibodyreversibly binds to one molecule of bap1 to form an affibody-bap1complex. you add 75pmoles of affibodyand 20pmoles of bap1 to a test tube with 3ml total volume. 36hours later, you measure the amount of affibody—bap1complex to be 4pmoles. a. assuming an association rate constant of 2x105m-1s-1, calculate the dissociation rate constant. b.how much additional affibodywould need to be added to achieve 10 pmoles of affibody—bap1complex at equilibrium? assume the affibodysource is highly concentrated such that you can neglect the change in volume upon affibodyaddition. c.recalculate the solution to part bif the affibodysource is present at500 nm, so that you cannot neglect changes in volume upon addition. what was the % error in part b?
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One molecule of affibodyreversibly binds to one molecule of bap1 to form an affibody-bap1complex. yo...
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