An enzyme that follows michaelis-menten kinetics has a km value of 15.0 μm and a kcat value of 221 s−1. at an initial enzyme concentration of 0.0100 μm, the initial reaction velocity was found to be 1.07×10−6 μm/s. what was the initial concentration of the substrate, [s], used in the reaction ?
Answers: 3
μM is the initial concentration of the substrate, [S], used in the reaction.![v=V_{max}\times \frac{[S]}{(K_m+[S])}=k_{cat}[E_o]\times \frac{[S]}{(K_m+[S])}](/tpl/images/0047/7743/d521f.png)
![V_{max}=k_{cat}[E_o]](/tpl/images/0047/7743/617d3.png)
μM/s![[K_m]](/tpl/images/0047/7743/99bb2.png)
= Michaelis constant = 15.0 μM
= Maximum rate achieved
= Catalytic rate of the system = 221 
![[E_o]](/tpl/images/0047/7743/e2395.png)
= Initial concentration of enzyme. =0.0100 μM![[S]=7.26\times 10^{-6}](/tpl/images/0047/7743/fcd8d.png)
μM
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