4. If you inserted your gene of interest in the plasmid above by ligation at the TsoI site, explain why these cells would not grow on agar plates containing ampicillin.

An example of internal feedback working to maintain homeostasis is your a. decreased respiration rate when you are studying b. decreased heart rate when you are sad c. increased respiration rate when your muscles need more oxygen d. increased heart rate when your lungs need more oxygen

Johnny rode his bike to a friend's house 4 blocks down the street in his neighborhood. he immediately rode back home once he realized his friend was not able to play. what was his displacement for the total bike ride? how did you determine this? what could we use as a reference point to determine he was in motion during his bike ride? why can you use it as a reference point

What function does the limb sever in each animal? how are the limb bones of the four animals similar? based on these similarities what can be inferred about the evolution of the four animals.