The correct sequence for western blotting is: 1. Proteins are denatured by sodium dodecyl sulfate (SDS), and by boiling.
2.Transfer proteins to the nitrocellulose membrane.
3. Performing the polyacrylamide gel electrophoresis (PAGE).
4. Incubate the nitrocellulose membrane to primary antibodies and secondary antibodies subsequently.

a. 1,2,3,4
b. 4,1,3,2
c. 1,3,2,4
d. 1,3,2,4

Subduction zones form when an oceanic plate collides with another oceanic plate or continental plate. the continental crust is lighter and less dense than oceanic crust. continental crust's density is approximately 2.7 grams per cubic centimeter. oceanic crust is thinner and the average density is about 3.3 cubic centimeters. when the two crustal plates converge the oceanic plate always bends and subducts beneath a continental plate. once the oceanic crust subjects, the rocks are subjected to changes in heat and pressure. because of this, we would expect to find rocks in the area of a subduction. a) clastic b) igneous c) metamorphic d) sedimentary

The united states uses which source of energy to produce almost 40 percent of its electricity

Name the tissue types that form the wall of each organ in questions 7-10