DNA polymerase I has 5′–3′ polymerase activity, 5′–3′ exonuclease activity, and 3′–5′exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase I may cause the enzyme to lose these activities.
Match the consequence of a loss‑of‑function mutation in DNA polymerase I to the corresponding lost activity.

a) Lost 5′–3′ polymerase activity
b) Lost 5′–3′ exonuclease activity
c) Lost 3′–5′exonuclease activity

1. No RNA primer removal during DNA replication
2. Decreased polymerase fidelity
3. No double helix denaturation
4. Unstable strand separation within the replication bubble
5. No DNA synthesis to fill gaps caused by removing RNA primers

Lost  5′ – 3′  polymerase activity = no DNA synthesis to fill gaps caused by removing RNA primers

Lost 5′–3′ exonuclease activity = no RNA primer removal during DNA replication

Lost 3′–5′exonuclease activity = decreased polymerase fidelity

Explanation:

DNA polymerase I wouldn't have the ability to synthesize new DNA strands if the 5' -> 3' polymerase activity was lost. Loss of the 5' --> 3' exonuclease activity would cause the RNA primers used to initiate replication to not be removed by DNA polymerase I. 3'–5' exonuclease activity participates in DNA repair, meaning that the loss of 3'–5' exonuclease activity would cause decreased polymerase fidelity.

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explanation: