Addition of causes the induction of the polymerase. This polymerase then binds to the T7 upstream of the rGFP gene, resulting in the of rGFP gene to produce rGFP mRNA. This mRNA is then to produce the rGFP protein .

Addition of HEAT causes the induction of the T7 RNA polymerase. This polymerase then binds to the T7 RNA POLYMERASE PROMOTER upstream of the rGFP gene, resulting in the TRANSCRIPTION of rGFP gene to produce rGFP mRNA. This mRNA is then TRANSLATED to produce the rGFP protein

Explanation:

T7 RNA polymerase is a warmth inducible compound that is initiated inside the nearness of a warmth source. This polymerase ties to a chose grouping alluded to as the T7 RNA Polymerase advertiser succession. It moves along the DNA grouping prompting the amalgamation of mRNA during a procedure alluded to as translation.  

This mRNA is changed over into a protein item through a procedure alluded to as interpretation.

idk what the options are but

these is a strong electrostatic attraction between the ions hence a lot of energy is needed to overcome this attraction

this is why the boiling points are so high