You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction, but instead are light purple. Your staining procedure was performed correctly. What is your best explanation as to why the bacteria have stained this way?

A. The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.
B. There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
C. The specimen did not undergo heat fixation before staining; therefore, the primary dye does not stick properly to the wall.
D. These are mutant bacteria which cannot be stained like other bacteria.

Where does all the water go? according to the environmental protection agency (epa), in a typical wetland environment, 39% of the water is outflow; 46% is seepage; 7% evaporates; and 8% remains as water volume in the ecosystem (reference: united states environmental protection agency case studies report 832-r-93-005). chloride compounds as residuals from residential areas are a problem for wetlands. suppose that in a particular wetland environment the following concentrations (mg/l) of chloride compounds were found: outflow, 60.4; seepage, 73.7; remaining due to evaporation, 26.4; in the water volume, 46.8. (a) compute the weighted average of chlorine compound concentration (mg/l) for this ecological system. (round your answer to one decimal place.) mg/l (b) suppose the epa has established an average chlorine compound concentration target of no more than 58 mg/l. does this wetlands system meet the target standard for chlorine compound concentration? yes. the average chlorine compound concentration (mg/l) is too high. yes. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is too high.

Construct at least two possible hypotheses for the student’s experiment.

You have trained year for a marathon. in your first attempt to run a marathon, you decide that you want to complete this 26-mile race in 4-5 hours. a. what is the length of a marathon in kilometers (1mile= 1.6 kilometers). b. what would your average speed have to be to complete the race 4.5 hours? give your answer in kilometers per hour.