Which of the following would be a reasonable experimental strategy to prevent translation of a particular prokaryotic mrna without targeting the ribosome? which of the following would be a reasonable experimental strategy to prevent translation of a particular prokaryotic mrna without targeting the ribosome? inhibit termination factors. inhibit the sigma factor. add suppressor trna molecules to the cell. mutate or remove the shine-dalgarno sequence on the mrna.

The correct answer is "Mutate or remove the Shine-Dalgarno sequence on the mRNA".

Explanation:

The Shine-Delgarno sequence is a fragment of bacterial and archaeal messenger RNA that helps to recruit the messenger RNA to the ribosome to initiate protein synthesis by translation. Mutating  or removing the Shine-Dalgarno sequence on the mRNA will prevent translation of a particular prokaryotic mRNA. Therefore, this is a reasonable experimental strategy to achieve this effect without targeting the ribosome, but the prokaryotic mRNA.

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure