Forward primer: 5'-agtctactcgtaaccggttacc-3' reverse primer: 5'-taaggcatcatggtaaccggtt-3' the two primers above have the melting temperatures between 55c-80c. what is the reason that these two primers will not bind effectively to the region of dna that we want to amplify?

Short answer is primers are partially complementary.

Explanation:

Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'

Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'

When we write reverse primer 5' to 3' we can easily see that

3'-TTGGCCAATGG---5' is complementary to the forward primers'

5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.

that scientist would be robert hooke

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