The technique of sanger sequencing takes advantage of the fact that dideoxynucleotides (nucleotides in which the 3′ hydroxyl group is absent) act as: a) palindromic sites. b) restriction sites. c) chain terminators. d) probes. e) none of the other answer options is correct.

Ans.

The Sanger sequencing or chain-termination method for sequencing involves use of ddNTPs (dideoxyribonucleotide triphosphates) to identify the sequence of nucleotides in a DNA segment. The ddNTPs are nucleotide derivatives that lack both the 2'- and 3'-hydroxyl group, hence called dideoxyribonucleotides. When a ddNTP is added to a growing chain of DNA, absence of 3'-hydroxyl group leads to termination of growing chain as this ddNTP cannot make phosphodiester bond with a NTP and DNA sequence can be identified.

Thus, the correct answer is option C).

phylogenetic tree is the tree of life or evolution tree. it shows the different branches of evolution. the sequence goes like tissues - coelom - backbone - endoskeleton - segmentation

the answer is: trichonympha lives in the gut of termites and digests cellulose eaten by the termite.

this is an example of a symbiotic association of a protozoan with its mode of association.

hope i

-lavira: )