Why did the government of South Carolina incorporate the city of Charleston ?

Explanation:

Charleston was the leading city in the South from the colonial era to the Civil War The city grew wealthy through the export of rice and, later, sea island cotton and it was the base for many wealthy merchants and landowners. ... Beginning during World War II, Charleston became a major naval base.

lack of interest from state and local governments.

this null effect is caused by a weak manipulation and ineffective internal validity. in a scientific study, as the one dr. morimoto is trying to perfom, internal validity refers to the confidence placed in the cause-effect relationship and to how well an experiment is done and able to eliminate confounding variables.