What was the main issue that led to the colonists wanting their independence

There are multiple answers for this but choose one of these. Taxation without representation, trial without jury, or quartering soldiers.

All three of these didn't bide well with the colonists. Hope this helps.

it is a part of john locke's natural rights popular during the age of enlightenment that people had inalienable rights to life, liberty and property. this was reflected by thomas jefferson in his declaration of independence as rights to life,liberty and the pursuit of happiness.

another line in the declaration of independence is that people have certain rights just because they are human. humans are capable of obeying the three natural rights john locke mentioned making them governable by these laws and therefore entitled to these rights as well.