A plasmid vector contains a single ecori site in the polylinker (multiple cloning site). an insert is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori and ligate the two. how many ecori site(s) are present in the recombinant dna molecule and how many band(s) would you see when you run your ecori digest of the recombinant dna on a gel? (there is no internal ecori site in the insert dna.)

answer: the revolutionary war, also known as the american war of independence, was a war from 1775 to 1783 between great britain and its thirteen colonies, which declared independence as the united states of america in 1776. people who wanted the colonies to gain independence and form their own country called the united states were referred to as patriots. they didn't feel that the treatment colonists received was fair, and one of the main issues was that they were being taxed without any representation in the british parliament. as dissatisfaction grew, they wanted to be a part of an independent country, which britain would have no ruler.

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answer: i am the bored too

explanation: i'm in a dumb class

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explanation: what is this supposed to mean, pls ask me some questions? and what’s year 7 naplan?

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yeah pritty girls

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