Allele specific oligonucleotides (aso) can be used to determine the different alleles present in a dna sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (pcr). The amplified dna is then spotted to filters and hybridized with probes that recognize single nucleotide differences. A sperm sample was collected from a single individual and dna was extracted from individual sperm. For each dna sample, four different regions of the genome were amplified using pcr (called a, b, d, and e). For each dna sample, aso probes were used to determine the allele present (either allele 1 or 2) for each region of the genome amplified. X indicates the allele is present and - indicates the allele is not present.

To the north of ginnungagap it was bitterly cold. (20) nothing was there but fields of snow and mountains made of ice. to the south of ginnungagap was a region where frost and snow were never seen. it was always bright, and was the home of light and heat. the sunshine from the south melted the ice mountains of the north so that they toppled over and fell into ginnungagap. there may be a mistake in the way the sentence is written. if you find a mistake, choose the answer that corrects the mistake. if there is no mistake, choose ‘correct as is.' a) correct as is. b) nothing was there but snow fields and mountains of ice. c) nothing was there but fields of snow and icey mountains. d) nothing was there but fields of snow and mountains of ice.