1. The directional movement of ions by facilitated diffusion through protein channels is determined by

The electrochemical gradient of the ion that is being transported.

Explanation:

Facilitated diffusion through protein is defined as a form of facilitated transport whereby passive movement of ions along concentration gradients are guided by the presence of other ions.

Thus, the electrochemical gradient of the ion being transported is what facilitates it

the answer is b i think

i just did an experiment like this in my science class. also the answer is yes, it will get separated because they are two opposites meaning if they are opposite they can not attract.

hope this .!