Fluorescence is a relatively faster phenomenon as compared with phosphorescence because of spontaneous emission of energy from s1 to s0. if it chooses the nonradiative path from s1 to a triplet state, t1 results in the slower decay of the process to reach the ground state s0. during this time interval, quenchers such as molecular oxygen can be involved in phosphorescence emission of the fluorophore. the process of quenching of the excited fluorophore in the triplet state by molecular oxygen can be one of the three different possible pathways such as energy transfer, electron transfer, and simple physical deactivation.
for the transition-metal complexes such as ruthenium, the energy-transfer mechanism is the most possible mechanism and it is dominated by the molecular collision thus limited by the diffusion of the quencher and is given by the stern−volmer relation. on the basis of the data derived from previous reports, a hydrophobic fluorophore of the ruthenium-based metal-ligand complex was selected for our sensing applications. to avoid the photobleaching effect of the fluorophore, a suitable substrate material has to be chosen and this provided broad adaptability to the specific needs. a fluorophore can be tethered or encapsulated onto a substrate material, and it can be in the form of a thin film or particle.

  sequence  is a continuous and distinctive band of  stars  that appears on plots of stellar  color  versus  brightness. these color-magnitude plots are known as  hertzsprung–russell diagramsafter their co-developers,  ejnar hertzsprungand  henry norris russell. stars on this band are known as  main-sequence stars  or  dwarf stars. these are the most numerous true stars in the universe, and include the earth's  sun.after condensation and ignition of a star, it generates  thermal energy  in its dense core region through  nuclear fusion  of  hydrogen  into  helium. during this stage of the star's lifetime, it is located on the main sequence at a position determined primarily by its mass, but also based upon its chemical composition and age. the cores of main-sequence stars are in  hydrostatic equilibrium, where outward thermal pressure from the hot core is balanced by the inward pressure of  gravitational collapse  from the overlying layers. the strong dependence of the rate of energy generation on temperature and pressure to sustain this balance. energy generated at the core makes its way to the surface and is radiated away at the  photosphere. the energy is carried by either  radiation  or  convection, with the latter occurring in regions with steeper temperature gradients, higher opacity or both.the main sequence is sometimes divided into upper and lower parts, based on the dominant process that a star uses to generate energy. stars below about 1.5 times the  mass of the sun  (1.5  m☉) primarily fuse hydrogen atoms together in a series of stages to form helium, a sequence called the  proton–proton chain. above this mass, in the upper main sequence, the nuclear fusion process mainly uses atoms of  carbon,  nitrogen  and  oxygen  as intermediaries in the  cno cycle  that produces helium from hydrogen atoms. main-sequence stars with more than two solar masses undergo convection in their core regions, which acts to stir up the newly created helium and maintain the proportion of fuel needed for fusion to occur. below this mass, stars have cores that are entirely radiative with convective zones near the surface. with decreasing stellar mass, the proportion of the star forming a convective envelope steadily increases. main-sequence stars below 0.4  m☉undergo convection throughout their mass. when core convection does not occur, a helium-rich core develops surrounded by an outer layer of hydrogen.in general, the more massive a star is, the shorter its lifespan on the main sequence. after the hydrogen fuel at the core has been consumed, the star  evolves  away from the main sequence on the hr diagram, into a  supergiant,  red giant, or directly to a  white dwarf.