The size of the human genome is 3.2x109bp. The 3.2kb long fragment is to be amplified by Polymerase Chain Reaction (PCR) using the human genome as a template. How many cycles of PCR is necessary for the amplified target sequence at least 50% (by mass) of the total DNA in the reaction mixture. Explain how you arrived to this number.

This gentleman has worsening bilateral hydronephrosis. he did not have much of a post void residual on his bladder scan. he is taken to the operating room to have a bilateral cystoscopy and retrograde pyelogram. cpt code: icd10-cm code: icd10-cm code: icd10-cm code

An african american young adult is admitted to the emergency department in sickle cell crisis with a report of 10/10 pain. the patient is known to several of the nurses and physicians in the department who have labeled the patient as a “drug seeker”. initial discussion post: identify one (1) intervention that can be taken by the rn to reduce the stigma and improve management of acute and chronic pain associated with sickle cell disease. does the intervention apply only to the patient? does it apply only to the interprofessional team? does it apply to both the patient and the members of the interprofessional team? explain and support your answer.

What are the benefits and challenges if you use in-house resources to manage accreditation versus obtaining an external consultant with specialization in health care accreditation?