You have isolated the mRNA for the human insulin gene and reverse transcribed it into cDNA. Explain how you could use this cDNA to make a recombinant plasmid and bacteria to commercially produce insulin to be used as a therapeutic drug. Include all of the following terms
in your answer (draw a diagram!):
Insulin gene (cDNA)
Plasmid
Host Cell
Recombinant Plasmid
Selective Media containing antibiotic
DNA Ligase
Sticky Ends
Restriction Enzyme
Sticky Ends Hybridize
Transform(ation)
Selection Using Antibiotic Resistance Gene
Lac Z gene
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You have isolated the mRNA for the human insulin gene and reverse transcribed it into cDNA. Explain...
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