Add fluo-3AM (lipid soluble) Cleave fluo-3AM to fluo-3 (water soluble)
Increase in fluorescence when bound to calcium
Calcium gets into cell through ion channels and via phospholipase Cβ

it will take around no amount of ruffe to knock over this 110lbs man because he semms to be around 300lbs. lol

answer:

cry and then a deep breath and breathe. let all your emotions out the way you need to, weather that crying a bunch or screaming do it then breath and remember it'll be okay.

explanation:

answer:

The application of an intermittent cryotherapy protocol after mild or moderate ankle sprain significantly reduced the level of subjective pain on activity, one week after the injury, compared with a standard protocol. There was no significant difference in terms of function, swelling, or pain at rest.