In the mid-1800s, travelers faced the greatest risk of attack by Native American groups on the California Trail.
Mormon Trail.
Oregon Trail.
Santa Fe Trail.

Santa Fe Trail.

Explanation:

The Santa Fe Trail was a 19th-century transportation route through central North America that linked Franklin, Missouri with Santa Fe, New Mexico. The route of the Santa Fe trailed crossed the territory of the Comanches, the north-western corner of Comancheria which is a Native American nation.

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genetics is the study of how dna works, the methodologies defining the new recent technologies allow scientist to have a panoramic view of the entire dna landscape, especially how genomes interact with their environment, including off course, factors outside their organisms.

thus is very possible the existence of both scenarios, where populations of isolated individuals have different genetic traits, and share traits related to genetic mutation, due rats can easily travel overseas using human transport channels. so scientists could get a vast number of data, including human interaction, evolution of rodents, health development, spread of infections, development of new organisms, how genomes evolve in different environments and so on.