What was a major difference between the knights of labor and the american federation of labor?

As in any developing country, labor and employing companies always had their differences. The Knights of Labor group was the first well recognized labor union and it was all inclusive, meaning almost anybody (women, blacks and but not Chinese) was welcomed. I think the Chinese were admitted at a later date. After the American Federation of Labor (AFL) was formed, the Knights of Labor fell out of favor and disappeared.
The major difference was the Knights of Labor formed the foundation upon which the AFL was born. Their demise was a natural evolution of the labor movement in this country. The Knights of Labor lacked good management and their inability to carry out successful strikes for their members caused their ranks to be recruited by the AFL.

That's true, it's a belief in one god

The dethroned emperor!