Which indigenous group was not one of the five nations most affected by the indian removal act?

Comanche

Explanation:

The Indian Removal act of 1830 authorized the relocation of Native Americans  westward of the Mississippi River and their ancestral lands were given to the whites. The five indigenous groups affected by the Act are; Chickasaw, Choctaw, Seminole, Cherokee, and Creek. About 4000 Cherokees died as a result of the forced eviction from their ancestral homes, this was later known as the Trail of Tears.

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