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b. the colonies must seek protection from other nations.

i believe it is the first choice (1.)

because the major theme is(“both the wild and civilization have rules.”)

and also as u said,( based on what buck learns within the call of the wild, "both the wild and civilization have rules".this is true of humans within society as well.

which sentence refers to a dynamic character?

b.

a man learns empathy after working with those he formerly judged.

hope this answers your question!