Identify the first step in preparing a spectrophotometer for use. A. Make sure all samples and the blank are ready for measurement.
B. Prepare a calibration curve.
C. Measure the absorbance of the blank.
D. Turn on the light source and the spectrophotometer.

D. Turn on the light source and the spectrophotometer.

Explanation:

A spectrophotometer is a machine used to measure the presence of any light-absorbing particle in a solution as well as its concentration. To prepare a spectrophotometer for use, the first step is to turn on the spectrophotometer and allow it to warm up for at least 15 minutes. After this is done, the next step will be to ensure that the samples and blank are ready. Next, an appropriate wavelength is set for the solute being determined. Finally, the absorbance is measured of both the blank and samples.