8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Avolume of 2.65 m3 of air in a rigid, insulated container fitted with a paddle wheel is initially at 264 k, 5.6 bar. the air receives 432 kj by work from the paddle wheel. assuming the ideal gas model with cv = 0.71 kj/kg • k, determine for the air the amount of entropy produced, in kj/k