8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Ariver flows from north to south at 8 km/h. a boat is to cross this river from west to east at a speed of 20 km/h (speed of the boat with respect to the earth/ground). at what angle (in degrees) must the boat be pointed upstream such that it will proceed directly across the river (hint: find the speed of the boat with respect to water/river)? a 288 b. 21.8 c. 326 d. 30.2

Avolume of 2.65 m3 of air in a rigid, insulated container fitted with a paddle wheel is initially at 264 k, 5.6 bar. the air receives 432 kj by work from the paddle wheel. assuming the ideal gas model with cv = 0.71 kj/kg • k, determine for the air the amount of entropy produced, in kj/k

A2-m rigid tank initially contains saturated water vapor at 100 kpa. the tank is connected to a supply line through a valve. steam is flowing in the supply line at 600 kpa and 300 c. the valve is opened, and steam is allowed to enter the tank until the pressure in the tank reaches the line pressure, at which point the valve is closed. a thermometer placed in the tank indicates that the temperature at the final state is 200°c. determine (a) the mass of steam that has entered the tank (b) the amount of heat transfer.