Amedium containing nutrient x in a 1000 liter reactor is sterilized. in the medium, you find an amount of contaminant microbe cell of 106 /l.
for the sterilization of this microbe, e0d=50 kcal/mol, and α=1028 min-1. the initial concentration of nutrient is 20 mg/l. the sterilization is performed at 121 oc. if you perform a sterilization time of 15 min, what is the unsuccessful fermentation probability? if under this condition, 20% nutrient will be degraded, calculate the degradation activation energy for nutrient x. assume α=106 min-1 for nutrient deactivation and r=1.987 cal/(mol k)