You have an aqueous solution in a test tube containing  mol/l of the peptide eee. with regard to e, the pka of the main carboxylic acid group is 2.19, the pka of the main amine group is 9.67, and the pka of the side chain group is 4.25. considering a ph of , derive an expression for the net negative charge per liter in the solution due to the peptide. as a separate question, do you expect the local pka of the side chain group of e in eee to be greater than, equal to, or less than 4.25? explain.