Describe what will happen if the sample spots are under the surface level of the solvent?

sup bro, im dumb, but this is what i found xd

"the ozone layer acts as a filter for the shorter wavelength and highly hazardous ultraviolet radiation (uvr) from the sun, protecting life on earth from its potentially harmful effects. when the sky is clear, there is an inverse relationship between stratospheric ozone and solar uvr measured at the earth's surface"