As discussed during the lecture, the enzyme HIV-1 reverse transcriptae (HIV-RT) plays a significant role for the HIV virus and is an important drug target. Assume a concentration [E] of 2.00 µM (i. e. 2.00 x 10-6 mol/l) for HIV-RT. Two potential drug molecules, D1 and D2, were identified, which form stable complexes with the HIV-RT. The dissociation constant of the complex ED1 formed by HIV-RT and the drug D1 is 1.00 nM (i. e. 1.00 x 10-9 mol/l). The dissociation constant of the complex ED2 formed by HIV-RT and the drug D2 is 100 nM (i. e. 1.00 x 10-7 mol/l).
1. Compute the total concentration of [D1]tot that is needed to bind 90% of the HIV-RT at the given concentration [E]tot. Provide your answer as a numerical expression with 3 significant figures in the unit mol/l. You do NOT have to consider competition betwwen the drugs D1 and D2! They are administered separately.
2. Compute the total concentration of [D2]tot that is needed to bind 90% of the HIV-RT at the given concentration [E]tot. Provide your answer as a numerical expression with 3 significant figures in the unit mol/l. You do NOT have to consider competition betwwen the drugs D1 and D2! They are administered separately.