For a given fluorophore, select the choice that correctly lists the processes of fluorescence, absorption, and phosphorescence in order from shortest to longest wavelength. a. absorption, fluorescence, phosphorescence
b. Fluorescence = phosphorescence, absorption
c. fluorescence, phosphorescence, absorption
d. phosphorescence, fluorescence, absorption
e. absorption, phosphorescence, fluorescence
f. absorption, fluorescence = phosphorescence

absorption, fluorescence = phosphorescence

Explanation:

Given a particular fluorophore, the wavelength of absorption of the fluorophore is always shorter. Both fluorescence and phosphorescence are kinds of photoluminescence.

Recall that both fluorescence and phosphorescence occur at a longer wavelength. The difference between the two is only in the time taken during the process. While fluorescence takes a shorter time to occur, phosphorescence takes a longer time to occur.

The major difference between fluorescence and phosphorescence is that change of spin occurs during phosphorescence but not fluorescence.

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