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Chemistry, 22.06.2019 05:30
A3.37-mg sample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0 ml. then 10.0 ml of this solution was placed in a 50-ml volumetric flask and treated with 5 ml of phenol solution plus 2 ml of sodium hypochlorite solution. the sample was diluted to 50.0 ml, and the absorbance at 625 nm was measured in a 1.00-cm cuvette and found to be 0.486. for reference, a standard solution was prepared from 10.0 mg of nh4cl (molar mass = 53.49 grams/mole) dissolved in 1.00 l of water. then 10.0 ml of this standard was placed in a 50-ml volumetric flask, treated in the same manner as the unknown, and the absorbance found to be 0.323. finally, a reagent blank was prepared using distilled water in place of unknown, it was treated in the same manner as the unknown, and the absorbance found to be 0.076. calculate the weight percent of nitrogen in the protein.
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Chemistry, 22.06.2019 10:10
Stage in which a star’s outer layers have started to cool and grow outward?
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Chemistry, 22.06.2019 12:30
How many grams of magnesium metal will react completely with 8.3 liters of 5.5m hcl? show all work
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Chemistry, 22.06.2019 14:30
What type(s) of intermolecular forces are expected between ch3ch2cooh molecules? dipole forces, induced dipole forces, hydrogen bonding
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