g Although graphical methods are available for accurate determination of the Vmax and KM of an enzyme-catalyzed reaction, sometimes these quantities can be quickly estimated by inspecting values of Vo at increasing [S]. Estimate the Vmax and KM of the enzyme catalyzed reaction for which the following data were observed. Vo is the initial reaction rate; Vmax is the maximum reaction rate; and KM is the Michaelis-Menten constant. [S] (M) Vo (microMolar/min) 2.5x10-6 28 4.0x10-6 40 1x10-5 70 2x10-5 95 4x10-5 112 1x10-4 128 2x10-3 139 1x10-2 140