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Chemistry, 25.02.2021 18:50 alexisbrad3107
Tris is a molecule that can be used to prepare buffers for biochemical experiments. It exists in two forms: Tris (a base) and TrisH (an acid). The MW of Tris base is 121.14 g/mol; the MW of TrisH is 157.6 g/mol (the extra weight is due to the Cl- counterion that is present in the acid). The Ka of the acid is 8.32 X 10-9. Assume that you have TrisH in solid form (a powder), unlimited 1M HCl, 1 M NaOH and distilled water. How would you prepare 1 L of a 0.02 M Tris Buffer, pH?
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