The following volumes of a standard 10.0 ppb F solution were added to four 10.00- mL aliquots of a water sample: 0.00, 1.00, 2.00, and 3.00 mL. Precisely 5.00 mL of a solution containing an excess of the strongly absorbing Al-acid Alizarin Garnet R complex was added to each of the four solutions, and they were each diluted to 50.0 mL. The fluorescence intensity of the four solutions were as follows: V mL Meter reading
0.00 68.2
1.00 55.3
2.00 41.3
3.00 25.8
a. Explain the working principle behind the analytical instrument used above and the chemistry of the analytical method.
b. The above instrument may have filter and spectro-flourometer. Describe the components of a filter fluorometer and a spectrofluorometer
c. Why is fluorescence instrument used above double beam in design?
d. Construct a standard additions plot for the data given above.
e. Calculate the concentration in ppb of F in the sample and standard deviation of the measured concentration.