What two things determine the potential energy of an object

The factors that affect an object's gravitational potential energy are its height relative to some reference point, its mass, and the strength of the gravitational field it is in.

You would be correct, boiling water does disinfect contamination.    the property of disinfecting means to kill  germs, bacteria and other pathogens or contamination  that might cause an infection or cause a person to become sickly. a couple methods of disinfecting are using soap, other cleaning supplies or by simply "boiling" the contaminate.  the reason why boiling water disinfects certain attributes is because some cells cannot survive the heat and pressure.

That is a lot of moles that you have