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Chemistry, 29.11.2020 19:30 tdbstonefamliy

List two different ways you could change the buffer to elute the bound protein(s) and achieve proper separation of the proteins. Select all that apply. Please explain why. a) add a buffer with pH=8.0

b) add a buffer with pH=5.0

c) decreasing the ionic strength of the buffer

d) increasing the ionic strength of the buffer

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