Chemistry, 30.10.2020 17:00 a897coleman

NADH and NAD are the reduced and the oxidized forms respectively of the coenzyme NAD. Solutions of NADH, but not NAD , absorb light at 340 nm. This property is used to determine the concentration of NADH in solution by measuring spectrophotometrically the amount of light absorbed at 340 nm by the solution. Explain how these properties of NADH can be used to design a quantitative assay for lactate dehydrogenase.

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NADH and NAD are the reduced and the oxidized forms respectively of the coenzyme NAD. Solutions of N...

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