Experiments performed with biological systems require a buffer that will not interfere with the stability and activities of the biological components. In 1966, Norman Good and his colleagues began a series of investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria include:a. pKa between 6 and 8b. highly water solublec. minimally permeable to biological membranesd. minimal effects of salt concentratione. minimla effect of temperature on pKaf. minimal effect of concentration on pKag. minimal interaction with metal ionsh. nontoxici. high chemical stabilityj. minimal absorbance between 240-700 nmFor each buffer below, choose the property that does NOT meet Good's criteria for biochemical buffers. If all of the buffer's properties satisfy the criteria for a Good's buffer, then select the last choice. Properties of phosphate buffer:pK2 at25C= 7.20 Δp Ka/C = -0.0028 Does not cross lipid bilayers Precipitate forms with Ca2+ ions All of the above properties fulfill the Good's buffer criteria Properties of HEPES buffer:pKa at 25'C =7.48 Δp Ka/C =-0.014 Water solubility limit is 2.25 M Low absorbance of light in I-JV region all of the above properties fulfill the Good's buffer criteria