There are only two naturally occurring isotopes of helium on earth. If you increase the % abundance of helium-3, does the % abundance of helium-4 change? Why or why not?

The chemical composition of soil varies with depth. an article in communications in soil science and plant analysis describes chemical analyses of soil taken from a farm in western australia. fifty specimens were each taken at depths 50 and 250 cm. at a depth of 50 cm, the average no3 concentration (in mg/l) was 88.5 with a standard deviation of 49.4. at a depth of 250 cm, the average concentration was 110.6 with a standard deviation of 51.5. find a 95% confidence interval for the difference in no3 concentrations at the two depths.