![subject](/tpl/images/cats/himiya.png)
Experiments performed with biological systems require a buffer that will not interfere with the stability and activities of the biological components. In 1966, Norman Good and his colleagues began a series of investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria included:
a. pKa between 6 and 8
b. highly water soluble
c. minimally permeable to biological membranes
d. minimal effects of salt concentration
e. minimal effect of temperature on pKa (pKa/°C)
f. minimal effect of concentration on pKa minimal interaction with metal ions non-toxic
g. high chemical stabilit
h. minimal absorbance between 240-700 nm
For each buffer, choose the property that does not meet Good's criteria for biochemical buffers If all of the buffer's properties satisfy the criteria for a Good's buffer:
Properties of acetate buffer: Properties of MOPS buffer:
1. highly water soluble pKa at 25°C 7.15
2. ApKJ°C=-0.0002 ΔpKJ°C =-0.013
3. pKg at 25°C = 4.76 absorbs light at <230 nm
4. does not cross lipid bilayer does not cross lipid bilayer
5. all the above properties fulfill all the above properties fulfil
the lipid criteria the lipid criteria
![ansver](/tpl/images/cats/User.png)
Answers: 2
![](/tpl/images/ask_question.png)
![](/tpl/images/ask_question_mob.png)
Another question on Chemistry
![question](/tpl/images/cats/himiya.png)
Chemistry, 21.06.2019 18:30
A6.10 m nacl can be made by adding [x]g of nacl to a container and making the volume of water up to the 1.00 l line
Answers: 1
![question](/tpl/images/cats/himiya.png)
Chemistry, 22.06.2019 08:30
Identify one disadvantage to each of the following models of electron configuration: -dot structures -arrow and line diagrams -written electron configurations type in your answer below. (answer) -dot structures do not show the distribution of electrons in orbitals and take up a lot of space. -arrow and line diagrams take up a lot of space and make it difficult to count electrons. -written configurations make it easy to lose count of electrons and do not show the distribution of electrons in orbitals.
Answers: 3
![question](/tpl/images/cats/himiya.png)
![question](/tpl/images/cats/himiya.png)
You know the right answer?
Experiments performed with biological systems require a buffer that will not interfere with the stab...
Questions
![question](/tpl/images/cats/biologiya.png)
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/istoriya.png)
History, 02.09.2021 04:00
![question](/tpl/images/cats/istoriya.png)
History, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/himiya.png)
Chemistry, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00
![question](/tpl/images/cats/en.png)
![question](/tpl/images/cats/fizika.png)
Physics, 02.09.2021 04:00
![question](/tpl/images/cats/mat.png)
Mathematics, 02.09.2021 04:00