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Chemistry, 05.05.2020 06:59 Aliyahh5673

Pure solid NaH2PO4 is dissolved in distilled water, making 100.00 ml of solution. 10.00ml of this solution is diluted to 100.00 ml to prepare the original phosphate standard solution. Three working standard solutions are made from this by pipetting 0.8ml, 1.5ml and 3.0 ml of the original standard solution into 100.00 ml volumetric flasks. Acid and molybdate reagent are added and the solutions are diluted to 100.00 ml. phosphate in the soft drink. You may assume that all these absorbance measurements have already been corrected for any blank absorbance. The absorbance of each is measured in the spectrophotometer. A 10.0 ml volume of soft drink is treated with acid and reagent and also diluted to 100.00 ml. Its absorbance is also measured. From the following data, plot a calibration line (absorbance vs. concentration), determine its slope and calculate the concentration of phospate in the original soft drink(mM).Data given:Mass of NaH2PO4 (mg) -- 536.4Absorbance standard 1 (0.8 ml) -- 0.1973Standard 2 (1.5 ml) --- 0.3701Standard 3 (3.0 ml) --- 0.7402sample (10.0 ml) -- 0.2230Calculate:Concentration of orignal phosphate standard (mM)concentration of standard 1(mM)concentration of standard 2(mM)concentration of standard 3(mM)Slope of calibration linethe concentration of phospate in the original soft drink(mM).

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