A large piece of biological tissue has to be kept at a temperature as low as possible without freezing (ice formation). The water in the tissue has several solutes dissolved in it and the effective molecular weight of the dissolved solutes is 50. The weight of all dissolved solutes combined is 10% of the weight of water. The latent heat of fusion of water is 335 kJ/ kg and the molecular weight of water is 18. What is the lowest temperature that this tissue can be stored at, without ice formation