In basic enzyme kinetics, a large Km indicates that the substrate binds A. Permanently B. Transiently C. Covalently D. with low specificity E. Weakly

D

Explanation:

Km value is a signature of the enzyme. It is the characteristic feature of a particular enzymes for a specific substrate. Km denotes the affinity of the enzyme for substrate. The lesser the numerical value of Km, the affinity of the enzyme for the substrate is more.

In the velocity x substrate graph in a fixed quantity if enzyme. As substrate concentration is increase, the velocity is also increasing at the initial phase but the curve fatten afterwards. This is because as more substrate is added, all enzymes molecules become saturated. Further increase in substrate cannot make any effect in the reaction velocity.

The maximum velocity is called Vmax. Km is the concentration of substrate that Vmax is half.

The larger the numerical value of Km, the lesser the enzyme binds the substrate

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