Adirect measurement of the standard freeâenergy change associated with the hydrolysis of atp is technically demanding because the minute amount of atp remaining at equilibrium is difficult to measure accurately. the value of î g â² â can be calculated indirectly, however, from the equilibrium constants of two other enzymatic reactions having less favorable equilibrium constants:
keil-270 k. g2=890 glucose 6-phosphate+ h2o â glucose+ p, eq1 atp + glucose â adp+ glucose 6-phosphate using this information for equilibrium constants determined at 25 "c, calculate the standard free energy of hydrolysis of atp

The answer to your question really depends on three things: how much water you have, how cold it is to start out, and how cold the things around it are. water actually freezes when it gets to 32 degrees fahrenheit (0 degrees celsius), but the time it takes to get there may be different.  let's start with the first. if you take two glasses, and fill one with a tiny bit of water, and the other about halfway, then put them both in the freezer, the one with less water will freeze first (you can try this at home, but i recommend using plastic cups and not glass ones).  now let's move on to the second part. let's say you have two glasses, and you fill one with really cold water that has been in the refrigerator, and the other with really hot water from the sink. if you put both of them in the freezer, the one that started out colder will freeze first.  for the third part, let's imagine that you have two glasses with the same amount of water in them, and the water is at the same temperature. imagine putting one outside on a really really cold day in georgia, and having a friend in alaska put one outside on the same day. since it would be so much colder in alaska, the glass of water there would freeze before yours.  so, if you took a tiny bit of really cold water in a glass, and put it outside on a cold day in alaska, it would freeze a lot faster than a big glass of hot water outside on a cold day in georgia.