Chemistry, 14.11.2019 20:31 SucMaDongShan
Using the henderson-hasselbalch equation, calculate the amount of hepes (sodium salt) and hepes (free acid) required to prepare 50 ml of a 100 mm buffer that is ph = 7.20. the pka of hepes is 7.55 at 20° c. the formula weight of the sodium salt is 260.31. the formula weight of the free acid is 238.31. weigh out the appropriate amounts of the hepes (sodium salt) and hepes (free acid), transfer to a 100 ml beaker, dissolve in deionized water to an approximate volume of 40 ml
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Using the henderson-hasselbalch equation, calculate the amount of hepes (sodium salt) and hepes (fre...
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