Acarbonic anhydrase generates h+ at a rate constant (kcat) of 10^(6) per second. assume that the rate constant of the enzyme is not affected by ph, and the initial ph of the reaction solution is 7.0. 1) what will be the ph of the reaction solution after 0.1 nm carbonic anhydrase catalyzes the reaction with unlimited substrate (co2) for 1 min? 2) what will be the ph of the reaction solution (0.1 nm carbonic anhydrase catalyzed the reaction with unlimited substrate (co2) for 1 min) if 100 mm tris (pka = 8.0) is included as buffer? (the initial ph of the solution is 7.0)

chemical energy is stored in the chemical bonds of atoms and molecules. it can only be seen when it is released in a chemical reaction. when chemical energy is released, the substance from which the energy came is often changed into an entirely different substance.

this chemical reaction releases energy in the form of light and heat. chemical energy is the potential energy stored in the arrangement of atoms within molecules. breaking chemical bonds requires energy, while forming new chemical bonds releases energy.

the atp molecule can store energy in the form of a high energy phosphate bond joining the terminal phosphate group to the rest of the molecule. in this form,energy can be stored at one location, then moved from one part of the cell to another, where it can be released to drive other biochemical reactions.

in the given question, the partial pressure is calculated by using the ideal gas equation, that is, pv = nrt

here, p is pressure, v is volume = 25 l, n is number of moles = 0.0104 moles, r is gas constant = -0.0821 l.atm / mol.k

t is temperature = 273 k

p = nrt / v

p = (0.0104 mol × 0.0821 l.atm / mol.k × 273 k) / 25 l

= 0.0093 atm

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