The protein lysozyme unfolds at a transition temperature of 75.5 oc, and the standard entropy of transition is 509 kj mol-1. calculate the entropy of unfolding of lysozyme at 25.0 oc, given that the difference in the constant-pressure heat capacities on unfolding is 6.28 kj k-1 mol-1, and can be assumed to be independent of temperature. hint: imagine that the transition at 25.0 oc, occurs in three steps: (i) heating of the folded protein from 25.0 oc to the transition temperature. (ii) unfolding at the transition temperature, and (iii) cooling of the unfolded protein to 25.0 oc . because the entropy is a state function, the entropy change at 25.0 oc is equal to the sum of the entropy changes of the steps.